Journal: Frontiers in immunology

Article Title: Genomic Multiple Sclerosis Risk Variants Modulate the Expression of the ANKRD55 - IL6ST Gene Region in Immature Dendritic Cells.

doi: 10.3389/fimmu.2021.816930

Figure Lengend Snippet: FIGURE 5 | Digital droplet PCR (ddPCR) quantification of gene transcript copy numbers in pDC, mDC, CD14+ monocytes, PBMC fraction minus monocytes (F-), and moDC. Analysis was performed in 350 pg cDNA (A, E, F) or 30 ng of cDNA (B–D). (C) ddPCR quantification of ANKRD55 transcript copy numbers in moDC treated with AM-580. (D) ANKRD55 copy numbers in moDC matured or not with IFN-g/LPS measured at 6 h after addition of stimulus. (F) Transcript levels of markers specific for mDC (CD1c) and pDC (IL3RA, CLEC4C).

Article Snippet: To verify the purity of each subpopulation, cells were analyzed by flow cytometry (MACSQuant® flow cytometer, Miltenyi Biotec) using antihuman CD1c (BCDA-1)-APC antibody (Miltenyi Biotec, Ref. 130-110-595) for mDC and CD303 (BDCA-2)-APC antibody (Miltenyi Biotec, Ref. 130-114-177) for pDC, as shown in Supplementary Figure S1.

Techniques:

Journal: The American Journal of Pathology

Article Title: Up-Regulation of the Chemokine CCL18 by Macrophages Is a Potential Immunomodulatory Pathway in Cutaneous T-Cell Lymphoma

doi: 10.1016/j.ajpath.2011.05.040

Figure Lengend Snippet: Antibodies Used for Immunofluorescence Staining of Skin Sections

Article Snippet: CD1a , Polyclonal , Rabbit IgG , Unlabeled , Atlas Antibodies.

Techniques: Immunofluorescence, Staining, Avidin-Biotin Assay, Plasmid Preparation

Journal: The American Journal of Pathology

Article Title: Up-Regulation of the Chemokine CCL18 by Macrophages Is a Potential Immunomodulatory Pathway in Cutaneous T-Cell Lymphoma

doi: 10.1016/j.ajpath.2011.05.040

Figure Lengend Snippet: Mature DCs do not significantly express CCL18. A: Immunofluorescence staining was performed on lesional sections for the markers indicated. Occasionally, CD1c+ DCs expressed CCL18 in PEP and MF patch or plaque or MF tumor stage. CD208+ cells did not co-express CCL18. B: CD209 was only occasionally co-expressed with CD1c in MF lesional skin. CD208 was mainly co-expressed by CD1a+ DCs in MF patch or plaque and MF tumor lesions. C: CD11c was expressed by CD1a+ cells in the tumor center. Some CD163+ macrophages also co-expressed CD11c. CD11c+ cells predominately located at the tumor invasion front were co-stained with CCL18.

Article Snippet: CD1a , Polyclonal , Rabbit IgG , Unlabeled , Atlas Antibodies.

Techniques: Immunofluorescence, Staining

Journal: Frontiers in Veterinary Science

Article Title: Dermal Microvascular Units in Domestic Pigs ( Sus scrofa domestica ): Role as Transdermal Passive Immune Channels

doi: 10.3389/fvets.2022.891286

Figure Lengend Snippet: The immunological structure of DMUs identified by IHC. (A) DMUs in pig cervical skin marked by CD68; (B) DMUs in pig cervical skin marked by CD1a; (C) DMUs in pig cervical skin marked by CD1c; (D) DDVPs in pig cervical skin marked by CD68; (E) DDVPs in pig cervical skin marked by CD1a; (F) DDVPs in pig cervical skin marked by CD1c; (G) CD68 + expression quantified by IOD in DMUs and DDVPs; (H) CD1a + expression quantified by IOD in DMUs and DDVPs; (I) CD1c + expression quantified by IOD in DMUs and DDVPs. (J) The anatomical location of DMUs in the skin and the structure of DMUs are presented in a schematic diagram. ns indicates no significant difference; * P < 0.05 indicates significant difference. Asterisks (*) point to microvasculature and arrows (→ ) point to capillary lymphatics; DMU, dermal microvascular unit; DDVPs, deep dermal vascular plexus; IOD, integrity optical density; scale bar (A–E) = 20 μm.

Article Snippet: Treated samples were blocked with 5% bovine serum albumin for 40 min (BSA, Boster Biological Technology, Wuhan, China) and incubated with rabbit anti-CD68 antibody (1:100, Boster Biological Technology, Wuhan, China), rabbit anti-CD1c antibody (1:100, Bioss Biological Technology, Beijing, China), and rabbit anti-CD1a antibody (1:100, Boster Biological Technology, Wuhan, China) overnight at 4°C, negative controls were set up with phosphate buffered saline (PBS, 0.1 M, pH 7.4) instead of primary antibody.

Techniques: Expressing